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Brown university graphpad prism license2/19/2023 The protein expression/localization of K Ca channels in the harvested atrial tissue samples, coronary microvessels, and primary cultured human coronary endothelial cells were assayed by Western blotting and immunohistochemistry. The in vitro relaxation response of pre-contracted arterioles was examined in the presence of the selective SK/IK opener NS309 (10 −9–10 −5 M). Coronary arterioles from the harvested right atrial tissue samples from diabetic and non-diabetic patients ( n = 8/group) undergoing cardiac surgery were dissected pre- and post-CP/CPB. In the current study, we further investigated the effects of diabetes on coronary arteriolar responses to the small (SK) and intermediate (IK) K Ca opener NS309 in diabetic and non-diabetic patients and correlated that data with the changes in the SK/IK protein expression/distribution in the setting of cardioplegic ischemia and reperfusion (CP) and cardiopulmonary bypass (CPB). We have recently found that diabetes is associated with the inactivation of the calcium-activated potassium channels (K Ca) in endothelial cells, which may contribute to endothelial dysfunction in diabetic patients at baseline.
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